rabbit polyclonal anti pcna antibody  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

doi: 10.64898/2026.01.27.701991

Figure Lengend Snippet: (A) Schematic of a sagittal view of the zebrafish brain highlighting the proximate location of the diencephalic posterior tubercular nucleus (PTN) and innervation of the Mauthner cells (M-cell). (B) Confocal projections of PCNA expression in a representative communal, dominant, and subordinate Tg( dat :egfp) zebrafish. Merged channel also shows DAPI nuclear staining in blue. Arrowheads point to PCNA expressing PTN somata. Scale bar = 20 μm. (C) Summary graph of the fraction of PTN neurons that express PCNA. Symbols represent individual samples; bars represent averages, error bars represent SEM. Dashed lines show pairwise comparison connecting each dominant to its subordinate counterpart. (Com n=6, Dom n= 8, Sub n= 8; One-way ANOVA, P=0.0029 , One-way ANOVA, Tukey’s Multiple Comparison post-test).

Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

Techniques: Expressing, Staining, Comparison

Journal: bioRxiv

Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

doi: 10.64898/2026.01.27.701991

Figure Lengend Snippet: (A) PC1 and PC2 are the first and the second principal components excluding social isolates. (B) Eigenvalues of the principal components excluding social isolates showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. (C) PC1 and PC2 are the first and the second Principal components excluding PCNA as a component. (D) Eigenvalues of the principal components excluding PNCA as a component showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. In A and C, points represent individual samples (n= 6 per social condition), different colors and symbols represent different groups. Ellipses represent 68% confidence intervals of core regions. Loading vectors represent original variables, the directions of arrows represent correlation between original variable and principal components, lengths represent association of original data to principal components. In B and D components are listed in descending order (highest to the lowest).

Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

Techniques:

Journal: Molecular Therapy Oncology

Article Title: Targeted nanoencapsulation of tunicamycin reduces toxicity while improving its therapeutic effectiveness in pancreatic cancer cells

doi: 10.1016/j.omton.2025.201047

Figure Lengend Snippet: Representative of H&E and PCNA IHC with TM concentrations in tumor and different organs (A) H&E staining of untreated and NP TM-treated KPC-cell-derived xenograft tumor (KPC-CDX) sections. Scale bars, 50 μm. (B) PCNA immunohistochemistry in untreated and NP TM-treated KPC-CDX sections. Bar graph showing the statistical difference of PCNA in untreated and untreated samples. Data represent mean ± SD. n = 5. Scale bars, 50 μm. (C) H&E staining in different organs of KPC-CDX mice upon NP TM treatment. 40×. Scale bars, 50 μm.

Article Snippet: Anti-rabbit IgG (# 70745) was purchased from Cell Signaling, USA; anti-mouse IgG (# sc-516102) from Santa Cruz, Inc, USA; CCN1 polyclonal antibody (#26689-1-AP) and Neuropilin-1 (NRP-1) from ProteinTech, USA; and pSTAT3 polyclonal antibody (#9145) and STAT3 polyclonal antibody, peIF4E and EIF4E antibodies, and PCNA rabbit polyclonal antibody (#13110T) from Cell Signaling, Inc., USA.

Techniques: Staining, Derivative Assay, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: The CD300c antibody CL7 suppresses tumor growth by regulating the tumor microenvironment in non-small cell lung carcinoma

doi: 10.3389/fonc.2025.1698857

Figure Lengend Snippet: CL7 reduces NSCLC cancer cell proliferation. IHC images and quantitative analyses were performed using the 10 mg/kg CL7 group, which served as the representative dose for downstream experiments. Immunohistochemistry analysis for proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, was performed on lung tissue sections from tumor-bearing mice (n = 6 per group). Images were acquired using a light microscope at 20× magnification. Three random fields were selected, and PCNA-positive cells were quantified using QuPath software. All data are presented as mean ± SEM. *p < 0.05 versus PBS group.

Article Snippet: The sections were then incubated overnight at 4°C with a rabbit polyclonal anti-PCNA antibody (1:200, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunohistochemistry, Marker, Light Microscopy, Software

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Spatially resolved multi-omics reveals the renal cortex-metabolic reprogramming of Shenhua Tablet for intervention on IgA nephropathy.

doi: 10.1016/j.phymed.2025.156742

Figure Lengend Snippet: Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing PCNA and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.

Article Snippet: The corresponding stain reagent was PAS Stain Kit (Solarbio, G1281), whereas the antibodies used included PCNA rabbit polyclonal antibody (1:200 dilution, Proteintech, 10,205–2-AP), Rabbit Anti-ODC1 antibody (1:200 dilution, Bioss, bs1294R), Col4 Polyclonal antibody (1:300 dilution, Bioss, bsm56208R), CD68 rabbit polyclonal antibody (1:200 dilution, Servicebio, GB113109), OX8 antibodies (1:200 dilution, boster, A02236–1), and HRP-conjugated goat anti-rabbit IgG (H + l) (1:200 dilution, ServiceBio, GB23303).

Techniques: Staining, Immunohistochemical staining, Expressing, Comparison